Schistosoma mansoni cercarial elastase (SmCE): differences in immunogenic properties of native and recombinant forms
نویسندگان
چکیده
منابع مشابه
The cercarial glycocalyx of Schistosoma mansoni
Cercariae, the freshwater stage of Schistosoma mansoni infectious to man, are covered by a single unit membrane and an immunogenic glycocalyx. When cercariae penetrate the host skin, they transform to schistosomula by shedding tails, secreting mucous and enzymes, and forming microvilli over their surface. Here the loss of the glycocalyx from cercariae transforming in vitro was studied morpholog...
متن کاملInvestigation of the Proteolytic Functions of an Expanded Cercarial Elastase Gene Family in Schistosoma mansoni
BACKGROUND Cercarial elastase is the major invasive larval protease in Schistosoma mansoni, a parasitic blood fluke, and is essential for host skin invasion. Genome sequence analysis reveals a greatly expanded family of cercarial elastase gene isoforms in Schistosoma mansoni. This expansion appears to be unique to S. mansoni, and it is unknown whether gene duplication has led to divergent prote...
متن کاملCercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
Schistosome parasites are the causative agents of schistosomiasis, a chronically debilitating disease that affects over 200 million people globally and ranks second to malaria among parasitic diseases in terms of public health and socio-economic impact (1-4). Schistosome parasites are trematode worms with a complex life cycle interchanging between a parasitic life in molluscan and mammalian hos...
متن کاملGlycomics analysis of Schistosoma mansoni egg and cercarial secretions.
The parasitic helminth Schistosoma mansoni is a major public health concern in many developing countries. Glycoconjugates, and in particular the carbohydrate component of these products, represent the main immunogenic challenge to the host and could therefore represent one of the crucial determinants for successful parasite establishment. Here we report a comparative glycomics analysis of the N...
متن کاملRecombinant Mycobacterium bovis BCG expressing the Sm14 antigen of Schistosoma mansoni protects mice from cercarial challenge.
The Sm14 antigen of Schistosoma mansoni was cloned and expressed in Mycobacterium bovis BCG as a fusion with the Mycobacterium fortuitum beta-lactamase protein under the control of its promoter, pBlaF*; the protein was localized in the bacterial cell wall. The rBCG-Sm14 strain was shown to be relatively stable in cultured murine and bovine monocytes in terms of infectivity, bacterial persistenc...
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ژورنال
عنوان ژورنال: Parasitology
سال: 2017
ISSN: 0031-1820,1469-8161
DOI: 10.1017/s0031182017000658